Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.
SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) should be stored at 4°C and is stable for 12 months.
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