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Goat Anti-Human IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free)
Secondary Antibodies
Secondary Antibody

Goat Anti-Human IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free) #96232

Citations (0)
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left column) or treated with Anti-human IgM (12 μg/mL, 10 min; right column), using Phospho-CD79A (Tyr210) (E8E9Z) Rabbit mAb #89660 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (bottom row), and co-stained with CD19 (SJ25C1) Mouse mAb (APC Conjugate). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from serum-starved Ramos and Daudi cells, untreated (-) or treated with anti-human IgM (12 µg/mL, 10 min; +), using Phospho-CD79A (Tyr210) (E8E9Z) Rabbit mAb #89660 (upper), CD79A (D1X5C) XP® Rabbit mAb #13333 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
To Purchase # 96232
Cat. # Size Qty. Price
500 µg

Supporting Data

MW (kDa)
Source/Isotype Goat 

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Description

B cells become activated when the B cell Receptor (BCR) interacts with its cognate antigen, resulting in activation of signal transduction cascades that promote proliferation, survival, and differentiation into antibody secreting plasma cells. BCR signaling can be interrogated experimentally through the use of antibodies that crosslink the BCR, which induces phosphorylation of numerous intracellular signaling molecules. These molecular events can be quantified at the single-cell level using applications such as flow cytometry and western blotting (1-3).
Endotoxin Less than or equal to 0.05 EU/μg, as determined by the LaL assay.

Product Usage Information

Working concentrations and length of treatment can vary depending on the desired effect.


Supplied in phosphate buffered saline (PBS), pH7.4, Low Endotoxin, Azide-free.


Store at –20°C. Aliquot to avoid multiple freeze/thaw cycles.

Specificity / Sensitivity

Goat Anti-Human IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free) detects the heavy chain of human IgM and has been cross-adsorbed against human IgG and IgA. This antibody may cross-react with IgM from other species.

Species Reactivity:


Source / Purification

Goat Anti-Human IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free) is produced by immunizing goats with human IgM, purified by affinity chromatography on human IgM covalently linked to agarose, and pepsin digested.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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