Goat Anti-Mouse IgG1 Antibody (AP Conjugate) #48253
- WB
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
1:2000 - 1:8000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Storage
Specificity / Sensitivity
Goat Anti-Mouse IgG1 Antibody (AP Conjugate) detects the heavy chain of mouse IgG1. This antibody has been cross-adsorbed against mouse IgG2a, IgG2b, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.
Species Reactivity:
Mouse
Source / Purification
Goat Anti-Mouse IgG1 Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG1. The Anti-Mouse IgG1 antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Background
The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
Limited Uses
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