Goat Anti-Mouse IgG1 Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG1. The Anti-Mouse IgG1 antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
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