Western blot analysis of mouse IgG subclasses (10 ng per lane) reduced and denatured in 1x SDS loading buffer with DTT using Goat Anti-Mouse IgG1, Fc gamma Specific Antibody (HRP Conjugate) (left) or Anti-mouse IgG, HRP-linked Antibody #7076 (right).
Affinity purified Goat Anti-Mouse IgG1, Fc gamma Specific antibody is conjugated to horseradish peroxidase (HRP). This product has been optimized for use as a secondary antibody in Western blotting applications.
Recommended Antibody Dilutions:
1:3000 - 1:5000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Supplied in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease- Free) and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 19
Goat Anti-Mouse IgG1, Fc gamma Specific Antibody (HRP Conjugate) detects the Fc portion of mouse IgG1 but does not cross-react with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. This antibody has minimal cross-reaction with human, bovine, and rabbit serum proteins. It may cross-react with immunoglobulins from other species.
Goat Anti-Mouse IgG1, Fc gamma Specific Antibody (HRP Conjugate) is produced by immunizing goats with mouse IgG1. The Anti-Mouse IgG1, Fc gamma Specific Antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
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