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Goat Anti-Mouse IgG2a Antibody (Biotin Conjugate)
Secondary Antibodies
Secondary Antibody

Goat Anti-Mouse IgG2a Antibody (Biotin Conjugate) #61663

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  1. WB
Western Blotting Image 1: Goat Anti-Mouse IgG2a Antibody (Biotin Conjugate)
Western blot analysis of mouse IgG subclasses (10 ng per lane) reduced and denatured in 1x SDS loading buffer with DTT using Goat Anti-Mouse IgG2a Antibody (Biotin Conjugate) (left) or Anti-mouse IgG (H+L), Biotinylated Antibody #14709 (right).
To Purchase # 61663
Cat. # Size Qty. Price
1 mg $ 325

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Supporting Data

MW (kDa)
Source/Isotype Goat 

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

Affinity purified Goat Anti-Mouse IgG2a antibody is conjugated to biotin. This product has been optimized for use as a secondary antibody in Western blotting applications.

Product Usage Information

Recommended Antibody Dilutions:

1:2000 - 1:8000

Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.


Supplied in PBS containing < 0.1% sodium azide. Store at 4°C. Do not aliquot the antibody.

Specificity / Sensitivity

Goat Anti-Mouse IgG2a Antibody (Biotin Conjugate) detects the heavy chain of mouse IgG2a. This antibody has been cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.

Source / Purification

Goat Anti-Mouse IgG2a Antibody (Biotin Conjugate) is produced by immunizing goats with mouse IgG2a. The Anti-Mouse IgG2a antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.


Biotinylated anti-species IgG conjugated antibodies enable signal amplification and are ideal for detecting lower expressing proteins.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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