Render Target: STATIC
Render Timestamp: 2024-11-06T11:02:20.689Z
Commit: 642d75590e907c0f7dfc7c6e3b846bcc0b02197c
XML generation date: 2024-06-07 17:48:07.829
Product last modified at: 2024-06-08T08:00:10.378Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Goat Anti-Mouse IgG2c Antibody (AP Conjugate) #88602

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    Affinity purified Goat Anti-Mouse IgG2c antibody is conjugated to alkaline phosphatase (AP). This product has been optimized for use as a secondary antibody in Western blotting applications.

    Product Usage Information

    Recommended Antibody Dilutions:

    1:2000 - 1:8000

    Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

    Storage

    Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Stable for 12 months when stored at -20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Goat Anti-Mouse IgG2c Antibody (AP Conjugate) detects the heavy chain of mouse IgG2c. This antibody has been cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.

    Species Reactivity:

    Mouse

    Source / Purification

    Goat Anti-Mouse IgG2c Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG2c. The Anti-Mouse IgG2c antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    Background

    The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
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