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Goat Anti-Mouse IgG2c Antibody (AP Conjugate)
Secondary Antibodies
Secondary Antibody

Goat Anti-Mouse IgG2c Antibody (AP Conjugate) #88602

Citations (0)
  1. WB
Western blot analysis of mouse IgG subclasses (10 ng per lane) reduced and denatured in 1x SDS loading buffer with DTT using Goat Anti-Mouse IgG2c Antibody (AP Conjugate) (left) or Anti-mouse IgG, AP-linked Antibody #7056 (right).
To Purchase # 88602
Cat. # Size Qty. Price
1 ml

Supporting Data

MW (kDa)
Source/Isotype Goat 

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Description

Affinity purified Goat Anti-Mouse IgG2c antibody is conjugated to alkaline phosphatase (AP). This product has been optimized for use as a secondary antibody in Western blotting applications.

Product Usage Information

Recommended Antibody Dilutions:

1:2000 - 1:8000

Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.


Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Stable for 12 months when stored at -20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Goat Anti-Mouse IgG2c Antibody (AP Conjugate) detects the heavy chain of mouse IgG2c. This antibody has been cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.

Species Reactivity:


Source / Purification

Goat Anti-Mouse IgG2c Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG2c. The Anti-Mouse IgG2c antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.


The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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