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Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate)

Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate) #86426

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Application Key:

IF-Immunofluorescence

Product Information

Product Description

This Cell Signaling Technology^® antibody is conjugated to FITC fluorescent dye under optimal conditions and formulated at 2.0 mg/mL.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:400 – 1:1600 should yield acceptable results for immunofluorescent assays.

When using rabbit Fc-directed secondaries, a higher concentration of primary antibody may be required. The optimal dilution of each primary antibody should be determined by titration.

FITC has relatively low photostability. Usage of an antifade reagent is recommended to preserve signal intensity.

Storage

Supplied lyophilized in a formulation of 0.02 M potassium phosphate, 0.15 M sodium chloride (pH 7.2), 10 mg/mL BSA, and 0.01% azide. Store lyophilized product for up to 12 months at 4C. Reconstitute lyophilized material with 1.0 mL dH2O and centrifuge until clear (if needed). Once reconstituted, the undiluted liquid product is stable for six weeks at 4ºC. For extended storage, aliquot and freeze at -20C or below to avoid multiple freeze/thaw cycles. Dilute to a working solution immediately prior to use.

Specificity / Sensitivity

Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate) reacts with the Fc portion of rabbit IgG. This antibody conjugate does not react with the Fab portion of rabbit IgG or human serum proteins.

Background

This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.

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