Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127
- WB
Supporting Data
| REACTIVITY | Rab |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Mouse |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Rab-Rabbit
Product Information
Product Description
Product Usage Information
Recommended Antibody Dilution:
1:2000
Storage
Protocol
Specificity / Sensitivity
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations.
Species Reactivity:
Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Background
Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.
Limited Uses
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