Tox/Tox2 (E6I3Q) & CO-0016-750 SignalStar™ Oligo-Antibody Pair #81643
- IHC
Order Information # 81643
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
Product Information
Product Usage Information
Application | Dilution |
---|---|
SignalStar™ Leica Bond | 1:50 - 1:200 |
SignalStar™ Manual | 1:50 - 1:200 |
Storage
Product Description
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3+ T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14).
- O'Flaherty, E. and Kaye, J. (2003) BMC Genomics 4, 13.
- Aliahmad, P. et al. (2012) Curr Opin Immunol 24, 173-7.
- Wilkinson, B. et al. (2002) Nat Immunol 3, 272-80.
- Aliahmad, P. et al. (2004) J Exp Med 199, 1089-99.
- Chi, T.H. et al. (2002) Nature 418, 195-9.
- Aliahmad, P. and Kaye, J. (2008) J Exp Med 205, 245-56.
- Aliahmad, P. et al. (2010) Nat Immunol 11, 945-52.
- Yun, S. et al. (2011) Immunol Lett 136, 29-36.
- Page, N. et al. (2018) Immunity 48, 937-950.e8.
- Alfei, F. et al. (2019) Nature 571, 265-9.
- Yao, C. et al. (2019) Nat Immunol 20, 890-901.
- Wang, X. et al. (2019) J Hepatol pii: S0168-8278(19)30301-0. doi: 10.1016/j.jhep.2019.05.015.
- Morimura, S. et al. (2014) Arch Dermatol Res 306, 843-9.
- Huang, Y. et al. (2014) Oncotarget 5, 4418-25.
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