Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.
Western blot analysis of extracts from HeLa cells, untransfected or transfected with control (-) or Aurora B (+) siRNA, demonstrating a reduction in Histone H3 phosphorylation. Aurora B was detected using Aurora B monoclonal antibody, phospho-Histone H3 was detected using Phospho-Histone H3 (Ser10) (6G3) Monoclonal Antibody #9706, and total Histone H3 was detected using Histone H3 Antibody #9715.
Western blot analysis of extracts from HeLa cells, untransfected or transfected with control (-) or targeted (+) siRNA. Aurora B was detected using Aurora B monoclonal antibody, Aurora A was detected using Aurora A/AIK Antibody #3092. The Aurora B monoclonal antibody confirms silencing of Aurora B expression, and the Aurora A/AIK Antibody is used to control for loading and specificity of Aurora B siRNA.
CST recommends transfection with 100 nM human-specific Aurora B siRNA 48 hours prior to cell lysis. See Protocol for transfection procedure.
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
SignalSilence® Aurora B siRNA from Cell Signaling Technology allows the researcher to specifically inhibit Aurora B expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.
Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).
The closely-related kinase Aurora B/AIM1 has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.