Buy Three, Get the Fourth Free! | Start Saving >>
6271
SignalSilence® Aurora B siRNA (Human Specific)
siRNA

SignalSilence® Aurora B siRNA (Human Specific) #6271

This product is discontinued

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected or transfected with control (-) or Aurora B (+) siRNA, demonstrating a reduction in Histone H3 phosphorylation. Aurora B was detected using Aurora B monoclonal antibody, phospho-Histone H3 was detected using Phospho-Histone H3 (Ser10) (6G3) Monoclonal Antibody #9706, and total Histone H3 was detected using Histone H3 Antibody #9715.

Learn more about how we get our images
Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected or transfected with control (-) or targeted (+) siRNA. Aurora B was detected using Aurora B monoclonal antibody, Aurora A was detected using Aurora A/AIK Antibody #3092. The Aurora B monoclonal antibody confirms silencing of Aurora B expression, and the Aurora A/AIK Antibody is used to control for loading and specificity of Aurora B siRNA.

Learn more about how we get our images
Fluorescent Detection

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.

Learn more about how we get our images

CST recommends transfection with 100 nM human-specific Aurora B siRNA 48 hours prior to cell lysis. See Protocol for transfection procedure.

Storage:

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

SignalSilence® Aurora B siRNA from Cell Signaling Technology allows the researcher to specifically inhibit Aurora B expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.

Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

The closely-related kinase Aurora B/AIM1 has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

  1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
  2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
  3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
  4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
  5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
  6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
  7. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
  8. Hauf, S. et al. (2001) Science 293, 1320-3.
Entrez-Gene Id
9212
Swiss-Prot Acc.
Q96GD4
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.