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SignalSilence® GRB2 siRNA I

SignalSilence® GRB2 siRNA I #13265

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® GRB2 siRNA I (+), using GRB2 Antibody #3972 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The GRB2 Antibody confirms silencing of GRB2 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

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CST recommends transfection with 100 nM SignalSilence® GRB2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

SignalSilence® GRB2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit GRB2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Growth factor receptor-binding protein 2 (GRB2) is a adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1 (1). The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of Tyr209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling (2).

  1. Pawson, T. and Scott, J.D. (1997) Science 278, 2075-80.
  2. Li, S. et al. (2001) EMBO J 20, 6793-804.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.

To Purchase # 13265S

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Product # Size Price
300 µl  (3 nmol) $ 256.0