Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® hnRNP E1 siRNA I (+) or SignalSilence® hnRNP E1 siRNA II #7987 (+), using hnRNP E1 Antibody #8534 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The hnRNP E1 Antibody confirms silencing of hnRNP E1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Learn more about how we get our images
CST recommends transfection with 100 nM SignalSilence® hnRNP E1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
SignalSilence® hnRNP E1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit hnRNP E1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
hnRNP E1 is a member of the hnRNP family of proteins that are involved in pre-mRNA processing and mRNA export, localization, stability, and translation (1-6). hnRNP E1 exerts a wide range of biological functions, such as transcriptional activation of mouse MOR gene expression (7), attenuation of alternative splicing of GHR pseudoexon expression (8), stabilization of collagen I and II (9), beta-globin (10), and androgen receptor (11) mRNAs, and regulation of translation of various genes including Dab2, ILEI, and Bag-1 (12,13). hnRNP E1 is ubiquitously expressed. Phosphorylation of hnRNP E1 affects its RNA binding affinity (13,14).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
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