Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.
Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with non-targeted (control) siRNA or transfected with Lamin A/C siRNA. Lamin A/C was detected using Lamin A/C Antibody #2032, and p42 MAPK was detected using p42 MAPK Antibody #9108. Lamin A/C Antibody confirms silencing of Lamin A/C expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity.
CST recommends transfection with 100 nM human specific Lamin A/C siRNA. Decreased Lamin A/C expression was seen 24-72 hours post-transfection. See Protocol for transfection procedure.
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
SignalSilence® Lamin A/C siRNA allows the researcher to specifically inhibit Lamin A/C expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from Cell Signaling Technology are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).
Previous studies have indicated that siRNA can effectively silence Lamin A/C expression (7,8). Reduction of Lamin A/C in HeLa cells does not affect cell growth, although the cells do show an unusuaual distribution of the inner nuclear membane protein emerin (8).
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