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6938
GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate)
WB & IP Reagents

GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) #6938

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Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 37
Isotype Rabbit 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology (CST) antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of GβL protein. The unconjugated GβL (86B8) Rabbit mAb #3274 reacts with human, mouse, rat, and monkey GβL protein. CST expects that GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) will also recognize GβL in these species.

Storage:

Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.

Specificity / Sensitivity

GβL (86B8) Rabbit mAb (Magnetic Bead Conjugate) detects endogenous levels of total GβL protein.

Source / Purification

GβL (86B8) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln210 of human GβL.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).

  1. Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302.
  2. Kim, D.H. et al. (2003) Mol Cell 11, 895-904.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

To Purchase # 6938