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3419
Streptavidin (Sepharose® Bead Conjugate)
WB & IP Reagents
Miscellaneous

Streptavidin (Sepharose® Bead Conjugate) #3419

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Immunoprecipitation Image 1: Streptavidin (Sepharose® Bead Conjugate)

Immunoprecipitation of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein using Myc-Tag (9B11) Mouse mAb (Biotinylated) #2084 and Immobilized Streptavidin (Bead Conjugate) (Lane 1) shows immunocomplexes pulled down using Streptavidin (Sepharose® Bead Conjugate). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using Myc-Tag (9B11) Mouse mAb (HRP Conjugate) #2040.

Immunoprecipitation Image 2: Streptavidin (Sepharose® Bead Conjugate)

Immunoprecipitation of extracts from NIH/3T3 cells using PTEN (138G6) Rabbit mAb (Biotinylated) #9583 and Streptavidin (Sepharose® Bead Conjugate) (Lane 1). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using PTEN (26H9) Mouse mAb #9556.

Immunoprecipitation Image 3: Streptavidin (Sepharose® Bead Conjugate)

Immunoprecipitation of extracts from Jurkat cells using Phospho-Akt (Thr308) (244F9) Rabbit mAb (Biotinylated) #3454 and Streptavidin (Sepharose® Bead Conjugate) (Lane 1). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106.

Product Usage Information

Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml pre-incubated with biotinylated primary antibody. See protocol for more details.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description

Streptavidin (Sepharose® Bead Conjugate) is useful for the precipitation of biotinylated proteins (1,2). Recombinant streptavidin is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads.

Background

Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).

  1. Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95.
  2. Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72.
  3. Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5.
  4. Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 3419S
Product # Size Price
3419S
400 µl  (40 immunoprecipitations) $ 118