Store Western Blot Stripping Buffer (5X) at room temperature. This product is stable for at least 12 months.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
ADDITIONAL REAGENTS (NOT SUPPLIED):
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
NOTE: This protocol begins after membrane exposure to film or digital imager. Best results are obtained if the membrane is not allowed to dry. Membranes can be stored in 1X TBST at 4℃.
NOTE: Quantitative comparison of targets before and after stripping is not recommended due to the removal of small amounts of membrane-bound protein with each round of stripping.
Protocol Id: 2564
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