Immunohistochemical analysis of paraffin-embedded human small intestine using VIP (D8J1V) Rabbit mAb (IHC Formulated) showing signal in nerve fibers of the muscularis externa.
Immunohistochemical analysis of paraffin-embedded mouse brain using VIP (D8J1V) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded mouse colon using VIP (D8J1V) Rabbit mAb (IHC Formulated) in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded rat colon using VIP (D8J1V) Rabbit mAb (IHC Formulated).
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
posted February 2010
revised March 2016
Protocol Id: 283
VIP (D8J1V) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total VIP protein.Species Reactivity:
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln133 of human VIP protein.
Vasoactive intestinal polypeptide (VIP) is a neuropeptide synthesized as a precursor that is processed to an active mature peptide of 28 residues (1). VIP is produced by neurons, endocrine, and immune cells and is expressed in many tissues, in agreement with its various biological functions (2). VIP acts through activation of two receptors belonging to the G protein-coupled receptor family, VPAC1 and VPAC2 (2) and elicits several effects such as vasodilation, regulation of smooth muscle cell contractility, and blood flow in the gastrointestinal track (3,4). In addition, VIP is involved in the regulation of T cell differentiation (6), and in immunosuppression (7,8).
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