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Resources results for "smad"

  • Products (9)
  • Resources (58)

Analyzing TGF-β Signaling in Cells to Spheroids: High-Throughput Methods Tested by Agilent and CST

3D spheroid models more accurately represent in vivo biological features and are being used in cancer research to mimic 3D tissue organization.

MH2 Protein Domain

A scientific resource for the MH2 protein domain containing information on structure, function, and domain binding to phospho-serine motifs during TGF-beta signaling.

TGF-β Signaling

Discover the TGF Beta signaling pathway and impact on cell growth & tissue homeostasis. Learn here the mechanisms behind this vital signaling cascade.

Poster: A Multiscale Approach to Quantitatively Evaluate the SMAD Signaling Pathway

Quantitative analysis of the SMAD signaling pathway using ELISA, high content imaging, and high-resolution confocal approaches were used to study the regulation of angiogenesis and cell proliferation.

A Multiscale Approach to Quantitatively Evaluate the SMAD Signaling Pathway

Contribution of Soluble Factors to EMT

A number of growth factors and cytokines can induce an Epithelial-Mesenchymal Transition (EMT) in tumor cells.

Resources

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338, and Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 are performance tested on extracts prepared from serum-starved cells that have been treated with 10ng/ml TGF-beta3 for 30 minutes.  It is possible that longer treatments (e.g., 24 hours) can lead to diminished signals for phospho-Smad2 and phospho-Smad3 because of Smad7 induction. 

​​Failure to include the appropriate serine/threonine phosphatase inhibitors can result in a diminished signal and/or inconsistent results between experiments. We always include sodium pyrophosphate (2.5mM final) and beta-glycerophosphate (1.0mM final) as serine/threonine phosphatase inhibitors in the lysis buffer for detection of the phospho-Smads. Our Phosphatase Inhibit…

Resources

  • Our Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 and Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820 are performance tested on extracts prepared from serum-starved cells treated with 50ng/ml BMP-2 for 30 minutes.
 

Drug Discovery Solutions Powered by CST

CST offers a broad catalog of top-quality products and services for many assay platforms, with tools and solutions to support scientists’ drug discovery research.

FYVE Protein Domain

A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.

Protein and Pathway Abbreviations

Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.

AACR 2023 Recap: Immunotherapy, CAR-T Cell Therapy Research Dominate

At AACR 2023, many presentations focused on topics including cancer immunology, CAR-T therapy, spatial biology, mRNA vaccines, and KRAS.

Angiogenesis Resources

Overview of Angiogenesis antibodies & related reagents, interactive pathway diagrams, and other technical resources.

Tools for Classifying Cells and Identifying Protein Localization

When do I want to classify cells? Why do I care where a protein localizes? These are two different questions that utilize different applications to answer.

Resources

The amino acid region surrounding the Ser463/465 phospho sites is well conserved in Smad1, 5, and 9. An alignment using the human sequences found in UniProt shows only one residue difference in this region. While we have performed testing to demonstrate that our Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820 binds phospho-Smad9, we have not performed similar testing with our Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516. Based on sequence conservation we would predict that #9516 may cross-react with phospho-Smad9, as we indicate in the Specificity/Sensitivity statement on our datasheet and product webpage. However, until we perform the necessary experiments we cannot guarantee phospho-Smad9 recognition using #9516.

Resources

Smad8 and Smad9 are often used in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there is a Smad8 in Xenopus that is not homologous. Therefore, we have decided that it would be less ambiguous to use Smad9 in our product names going forward.  The specificity of the Smad1/5/9 antibodies has not changed.
 

Tools for Modulating Cells

Cell behavior is a tightly regulated process where various signals work together to elicit a specific response. Unraveling these mechanisms allows us to understand how cells are regulated in a normal state.

Ubiquitin Ligase Table

The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).

Resources

Smad8 and Smad9 are often used interchangeably in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there is a Smad8 in Xenopus that is not homologous to Smad9. Therefore, to avoid ambiguity, we use Smad9 in our product names.

Pancreatic Cancer: Targeting Novel Drivers in the Tumor Microenvironment

There's a need for new methods to diagnose and treat pancreatic cancer. Biomarkers like CA 19-9 and MUC5AC are being explored as prognosis indicators.

Developmental Biology Signaling

Overview of stem cell markers, lineage markers, development and differentiation signaling networks at Cell Signaling Technology.

Stem Cell & Lineage Markers

Overview of stem cell markers, lineage markers, development and differentiation signaling networks at Cell Signaling Technology.

Tools for Monitoring Cell State

Cell states can be monitored using markers correlating to your process of interest. These assays and antibodies can be used to detect cell viability, senescence, proliferation, apoptosis, cytotoxicity and oxidative state.

Fibrosis

Products and Related Resources for Fibrosis SARS-CoV-2 Research

Resources

Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the cytoplasm.

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, and Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338 recognize phosphosites at the carboxy-terminus of Smad2 that are phosphorylated in response to TGF-beta treatment. Phospho-Smad2 (Ser245/250/255) Antibody #3104 instead recognizes a cluster of phosphosites in the Smad2 linker region that are phosphorylated by MAP kinase.  
 

Who am I: Can a cellular identity crisis lead to cancer progression?

Can a cellular identity crisis lead to cancer progression?

Melanoma: Aberrant Signaling Pathways and Therapeutic Approaches

Melanomas account for 90% of skin cancer deaths. A big contributor to this: the lack of effective, durable treatments for unresectable, metastatic melanoma.

Novel Therapeutic Approaches for Diffuse Large B-Cell Lymphoma

Lymphoma is a cancer of the lymphatic system, a key part of the body’s immune system. Treatment options & patient prognoses differ based on lymphoma type.

Analyzing PTM Activation State Using Western Blotting

Post-translational modifications result in different protein behaviors. Western blotting can be used to assess protein PTM states using antibody pairs.