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CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment.
Products and Related Resources for Adaptive Immune Response SARS-CoV-2 Research
Overview of stem cell markers, lineage markers, development and differentiation signaling networks at Cell Signaling Technology.
Learn more about stem cell pathways and the transcription factors Oct-4, Sox2, and Nanog are essential for maintaining the pluripotent state of stem cells.
Chromatin Immunoprecipitation Workflow Solutions for ChIP-qPCR and ChIP-seq
CUT&Tag delivers quality NGS data even if the signal from the Bioanalyzer or TapeStation System is low and the DNA library yield is low.
Maximize your research with our CUT&RUN Kit. Streamline chromatin profiling with our efficient, user-friendly tools designed for deeper, more insightful analyses.
CUT&RUN Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
With the appropriate salt concentration in the tagmention buffer, we don't observe bias toward euchromatin or heterochromatin in our assays. In CUT&Tag, the target-specific antibody and the secondary antibody recruit the pAG-Tn5 and direct the tagmentation to the chromatin directly adjacent to the target-specific antibody, whether in euchromatin or heterochromatin. The active tethering of pAG-Tn5 to the chromatin allows for tagmentation to occur even in less accessible heterochromatin regions. If CST scientists observe non-specific tagmentation at open chromatin regions when they are validating an antibody for CUT&Tag, then the antibody is not approved for use in the CUT&Tag assay.Our CUT&Tag Assay Kit #77552 has been shown to work well with antibodies against active, accessible euchromatic histone modifications (see Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751) and activating transcription factors (see TCF4/TCF7L2 (C48H11) Rabbit mAb #2569), as well as repressive, …
Learn how CUT&Tag cuts down your chromatin profiling experiment time. You’ll get the same great data with a fraction of the DNA library prep time and improved signal-to-noise.
Explore the intricate Wnt Beta Catenin pathway & its role in cell development and disease. Learn more about the signaling cascade of this crucial pathway.
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.
A number of growth factors and cytokines can induce an Epithelial-Mesenchymal Transition (EMT) in tumor cells.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Fostering mentorship, application-based learning, and a love of science are all part of the mission of the CST internship program. Students interning with us are partnered with CST Mentors.
Expert-reviewed signaling pathway providing a current overview of adherens junction dynamics and links to products by Cell Signaling Technology.
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
The two dominant bands detected by our TCF4/TCF7L2 antibodies (#2565, #2569, and #2953) represent different splice variants of TCF4/TCF7L2 [see Weise, A. (2010) Nucleic Acids Res 38, 1964-81 (PMID: 20044351; https://pubmed.ncbi.nlm.nih.gov/20044351/)].
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
Cancer cells can revert to a pre-differentiated, stem-cell-like phenotype, allowing uninhibited cellular division and other metabolic adaptations that enable survival in adverse conditions. Two key signaling components enable replicative immortality, Hippo and WNT.