Guide for ChIP and ChIP-seq

Sonication and Enzymatic Protocols.


Technical Support

Our scientists are at the bench daily to produce and validate our antibodies, so they have hands-on experience and knowledge of each antibody’s performance.

Contact a Scientist

For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.

Product specific protocols will be linked from matching product web pages.

Western Blotting & Immunoprecipitation


Immunohistochemistry Troubleshooting


Flow Cytometry

Flow Cytometry Troubleshooting

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Troubleshooting

Sandwich Immunoassays (ELISA)

In-Cell Western