Render Target: SSR
Render Timestamp: 2024-10-09T13:05:04.377Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
< Back to Support Article Search Results

Which total AMPKα antibody do you suggest for my western blot experiment?

The choice of AMPKα total antibody depends on the subunit of interest and the secondary antibody to be used. Cell Signaling Technology (CST) has five AMPKα antibodies that detect total protein: 

  • AMPKα Antibody #2532
  • AMPKα (D5A2) Rabbit mAb #5831
  • AMPKα (D63G4) Rabbit mAb #5832
  • AMPKα (F6) Mouse mAb #2793
  • AMPKα (23A3) Rabbit mAb #2603
It is important to note that AMPKα (D63G4) Rabbit mAb #5832 has been shown to recognize only AMPKα1 and not AMPKα2.

A side-by-side comparison of these five AMPKα antibodies has been performed (data available upon request). It revealed that the mouse-derived antibody, AMPKα (F6) Mouse mAb #2793, produces the strongest signal in western blotting applications. Of the rabbit-derived AMPKα antibodies, #5831 and #5832 show the strongest signals for western blotting. The polyclonal antibody, #2532, shows the weakest signal for western blotting applications.

Please note that signal strength in western blot does not necessarily correlate with antibody performance in other applications. If you have questions about choosing an AMPKα for an application other than western blot, please reach out to CST Technical Support
 

Last updated: September 12, 2024

Was this article helpful?

Technical Support

Email: [email protected]

Send Us a Message

Call: 877-678-8324

Customer Support

Email: [email protected]

Send Us a Message

Call: 877-616-2355

Fax: 866-432-6112

Contact Sales

Email: [email protected]

Send Us a Message

Call: 877-616-2355