Can I freeze my cells and pool them for a later CUT&RUN experiment?

Yes, you may freeze your cells and pool them for a later CUT&RUN experiment. However, we would suggest a light fixation using 0.1% formaldehyde for 2 min before freezing the cells, in case the protein-DNA association inside the nuclei changes during the freeze-thaw of the cells.

Last updated: September 12, 2024