Can I use RIPA Buffer (10X) #9806 as a replacement buffer for the Lysis/Binding/Wash Buffer #11524 in the GTPase kits?

We do not recommend using RIPA Buffer (10X) #9806 as a replacement buffer for Lysis/Binding/Wash Buffer #11524 in our GTPase Detection Kits. The RIPA buffer contains sodium dodecyl sulfate (SDS), which is considered a strong ionic detergent, while our GTPase kits utilize a mild, non-ionic detergent. We have not tested harsh detergents like SDS with these kits and, therefore, cannot recommend this substitution as we do not have a protocol or in-house data to support the functionality.

Last updated: September 18, 2024