Do your AIF Antibody #4642 and AIF (D39D2) XP® Rabbit mAb #5318 detect mitochondrial and mature/soluble AIF?

Both our AIF Antibody #4642 and our AIF (D39D2) XP Rabbit mAb #5318 are predicted to detect full-length AIF [amino acids 1-613 (predicted to be 67 kDa)], mitochondrial AIF [minus amino acids 1-54 (predicted to be 62kDa) and anchored to the inner mitochondrial membrane], and soluble/mature AIF [minus amino acids 1-101 (predicted to be 57kDa) and released to the cytoplasm to be translocated to the nucleus]. Although the apparent molecular weight is reported as 67kDa, the dominant band shown in our western blot images most likely represents mitochondrial AIF (predicted to be 62 kDa). Regrettably, the western blot images shown on the product webpages/datasheets do not include extracts prepared from apoptotic cells. However, multiple publications show detection of mature AIF by these antibodies.

Last updated: February 23, 2024