Does Cell Lysis Buffer (10X) #9803 work with the Bradford assay?

Cell Lysis Buffer (10X) #9803 will interfere with the Bradford protein assay. This is because it contains detergent. To make the assay somewhat compatible, dilute your protein quantitation samples at least 10-fold with water or PBS to reduce the amount of detergent and consequentially lower the interference. This ten-fold dilution may still yield background absorbance. We recommend employing an alternative protein assay that is compatible with #9803, such as the BCA Protein Assay Kit #7780.

Last updated: September 12, 2024