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Does Cell Lysis Buffer #9803 lyse whole cell membranes, nuclear membranes, or other organelle membranes?

Our Cell Lysis Buffer #9803 will lyse whole cell membranes. Please keep in mind that sonication is crucial to maximize protein recovery when using Cell Lysis Buffer #9803 as sonication will ensure ample nuclear rupture and DNA shearing. Please refer to our recommended lysing protocol for Cell Lysis Buffer #9803 on the product webpage: http://www.cellsignal.com/products/9803.html. If you are looking to separate out fractions (cytoplasmic, membrane/organelle, nuclear/cytoskeletal) when lysing your cultured cells, feel free to consider our Cell Fractionation Kit (#9038).

Last updated: February 26, 2024

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