Does CUT&RUN show a bias toward euchromatin or heterochromatin?

We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly adjacent to the antibody, whether that be in euchromatin or heterochromatin. In this case, the active tethering of pAG-MNase to the chromatin allows for digestion to occur even in less accessible heterochromatin. Our CUT&RUN Assay Kit #86652 has been shown to work well with antibodies against active, accessible euchromatic histone modifications (see Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751) and activating transcription factors (see NF-kB p65 [D14E12] XP^® Rabbit mAb #8242), as well as repressive, inaccessible heterochromatic histone modifications (see Tri-Methyl-Histone H3 [Lys27] [C36B11] #9733) and polycomb repressor complex proteins that are associated with inactive heterochromatin (see EZH2 [D2C9] XP^® Rabbit mAb #5246 and RING1B [D22F2] XP^® Rabbit mAb #5694).

Last updated: May 5, 2023