Does the CST CUT&Tag kit work with isolated nuclei?

Our CUT&Tag kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While our kit works with pre-isolated nuclei because the Concanavalin A beads bind to glycoproteins on the surface of both cells and nuclei, we haven't found that pre-isolation of cell nuclei helps to increase the CUT&Tag signal. Pre-isolation of cell nuclei should only be necessary for cells that are not efficiently permeabilized by digitonin, such as yeast or plant cells, which both contain cell walls.

Last updated: February 29, 2024