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Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?

No, the use of Fc block is not required when using F(ab') or F(ab')2 antibody fragments in live cell flow cytometry assays, as removal of the Fc domain eliminates the interaction between rabbit IgG and any Fc receptors expressed on the cell surface that would cause off-target binding.

Last updated: May 23, 2024

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