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How do citrate and EDTA retrieval compare (in general)?

We have performed retrieval comparisons with a number of antibodies and found, generally, that EDTA tends to result in stronger signals, both specific and potentially non-specific. If antibodies that are optimized with citrate retrieval are used with EDTA, further titration may be required in order to achieve an optimal signal. Conversely, use of citrate in some instances where EDTA is recommended could contribute to a weaker signal.

Last updated: March 7, 2023

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