How much chromatin do I need for my ChIP experiment?

In our experience, digesting 4x10^6 cells yields 10-20 ug of chromatin, which is why this is mentioned in our ChIP FAQ. However, as stated in the protocols for our SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005, approximately 5ug - 10ug chromatin is required for ChIP depending on your target. In our experience, for histone (PTM) ChIP, as low as 1-2 ug of chromatin DNA is required per IP. For transcription factor ChIP, a minimum of 5-8 ug chromatin DNA is required per IP. For co-factor ChIP, a minimum of 10 ug chromatin is necessary per IP. This is why all of our validated antibodies have optimal dilutions based on 10ug per IP.

Last updated: September 12, 2024