How much CUT&RUN DNA is required for NG-seq analysis?

The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction. Our SimpleChIP^® ChIP-seq DNA Library Prep Kit for Illumina^® #56795 will work with starting DNA as low as 0.5 ng. You can also increase the number of PCR cycles up to 20 to increase amplification of the library if the starting amount is too low.

Last updated: May 8, 2023