How much sample is required for a PTMScan® experiment?

We recommend using 10 mg of protein per immunoaffinity purification (IAP) sample for our standard PTMScan kits. For the PTMScan HS product line, 1 mg of starting material is optimal. The minimum sample amount is dependent upon the cell line/tissue and the endogenous levels of post-translational modifications (PTMs). If less protein is used, fewer identifications could be expected.

Last updated: September 12, 2024