I am not getting a signal for HIF-1alpha. How can I improve the signal?

It is very difficult to detect HIF-1alpha under normoxic conditions as the protein is quickly degraded. To see strong signal, it is necessary to induce the expression of the protein by either stressing the cells under hypoxic conditions or treating with a hydroxylase inhibitor such as cobalt chloride.

Last updated: October 6, 2025