I cannot complete the BrdU cell proliferation assay in the given time. Is there a step where I can stop?

We recommend that you complete the BrdU cell proliferation assay as soon as you have the time. However, if you are unable to complete the experiment during the recommended time, you can complete the experiment through Step B: BrdU Incorporation and then pause. To do this, please follow the steps below:
1. Add the fixing/denaturing solution and incubate for 30 minutes (Part C: BrdU Assay Step 1).
2. Remove your solution and dry your plate at room temperature for 30 minutes. 
3. Cover plates with parafilm and store at 4C.
It is important to make sure your plate is completely dry before you cover with the parafilm and store it. Dry plates can be stored for up to 1 month at 4C, however, the signal of the assay may decrease slightly.

Last updated: September 12, 2024