Is there a maximum number of cells that can be used in a single CUT&RUN assay?

We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, one can increase the number of cells to 250,000 per assay without scaling up beads, antibodies, or buffers.

Last updated: April 25, 2024