Should I spike in IgG2A or IgG1 when using the SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit #69486?

It should not matter whether you spike in IgG2a or IgG1 antibodies when using the SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit #69486, as the kit is measuring the ability of the sample to block the ACE2-RBD interaction and is not directly measuring the antibodies. Specifically, this kit is designed to detect blocking antibodies present in human serum or plasma samples.

Last updated: August 3, 2025