What can cause false positives using the Senescence β-Galactosidase Staining Kit #9860?

The pH of the staining solution is critical and a pH below 6.0 is the most common cause of false positives when using the Senescence β-Galactosidase Staining Kit #9860. False positives may also be seen with:

• Serum-starved cells
• Contact inhibited cells
• Confluent or over-confluent cells 
• Increased lysosomes unrelated to senescence
• Specific cell lines

Last updated: September 12, 2024