What conditions do you recommend for primary antibody incubation in my IF experiment?

We recommend dilution of the primary antibody into PBS containing 1% BSA and 0.3% Triton X-100. BSA acts as a carrier protein and improves antibody stability in dilute environments while Triton X-100 aids in ensuring maximum cell coverage. We recommend overnight incubation to allow maximum time for antibody binding. If the incubation time needs to be shortened, we generally recommend at least 2 hours at 37C but performance cannot be guaranteed.

Last updated: March 14, 2024