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What is the serum concentration of Normal Goat Serum #5425?
This product is purified from goat blood and is therefore 100% goat serum. If you are using it as a blocking buffer, you can dilute it as follows:
Blocking Buffer: 1X PBS + 5% normal serum + 0.3% Triton™ X-100
To prepare 10 mL, add 0.5 mL normal serum from the same species as the secondary antibody (e.g., Normal Goat Serum #5425 ) to 9.5 mL 1X PBS and mix well. While stirring, add 30 µL Triton™ X-100.
Last updated: September 12, 2024
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