Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IF-IC with mouse samples. These are:

• Cleaved Caspase-3 (Asp175) Antibody #9661
• Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664
• Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579

We recommend #9664 for mouse samples, as we have observed non-specific labeling in certain sub-types of healthy cells in fixed-frozen mouse tissues (e.g., pancreatic alpha-cells) and nuclear background in rat samples with Cleaved Caspase-3 (Asp175) Antibody #9661 and Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.
 

Last updated: September 12, 2024