Which mouse-reactive PD-L1 antibody is best for western blotting?

Cell Signaling Technology currently offers four mouse-reactive antibodies targeting PD-L1: 

• PD-L1 (F3Z1T) Rabbit mAb #73552
• PD-L1 (D4H1Z) Rabbit mAb #60475
• PD-L1 (D5V3B) Rabbit mAb #64988
• PD-L1 (F4G6U) Rabbit mAb #57396

Of these antibodies, only #60475 and #73552 are approved for western blotting purposes, while #64988 is approved for use in immunohistochemistry applications, and #57396 is approved for use in flow cytometry applications. 

Our in-house testing shows that #73552 exhibits superior sensitivity compared to #60475 when binding the extracellular domain of PD-L1. 

Last updated: June 2, 2025