Why did I lose all my cells after the InTraSeq™ protocol?

When starting Step B. Blocking (Day 2) and continuing until the end of the protocol, it is crucial to centrifuge at 850 x g (not RPM). Centrifuging at lower speeds will result in cell loss.

Additionally, starting with Step C. Immunostaining (Day 2), it is essential to ensure the cell pellet is submerged in ~40 μL of fluid in every step that requires removing and discarding the supernatant. Attempting to discard all of the supernatant will result in the loss of cells.

Last updated: September 18, 2024