Why do I see a band at 70kD when western blotting with your PTEN (D4.3) XP Rabbit mAb #9188 and PTEN (138G6) Rabbit mAb #9559?

In humans, there is a PTEN long isoform (isoform alpha) that can potentially be detected by our PTEN (D4.3) XP Rabbit mAb #9188 and PTEN (138G6) Rabbit mAb #9559 based on sequence homology. There is also a paper that cites #9559 specifically detecting the long isoform of PTEN. [see H. Liang et. al (2014) Cell Metab. 19, 836-48, PMID: 24768297, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097321]

Last updated: September 12, 2024