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Why do I see a doublet when using the Bcl-xL (54H6) Rabbit mAb #2764 for western blot?
The Bcl-xL (54H6) Rabbit mAb #2764 recognizes a portion of the human Bcl-xL sequence which is retained in all three isoforms of Bcl-xL. Please see UniProt for the precise isoform sequences:
https://www.uniprot.org/uniprot/Q07817#sequences
The doublet is most likely Bcl-xL and Bcl-xB, as Bcl-xS has a lower predicted molecular weight (19 kDa versus 25-26 kDa).
Last updated: February 23, 2024
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