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What are the two bands seen in western blot analysis using ANKRD15 (E9I4I) Rabbit mAb #69953?

When using ANKRD15 (E9I4I) Rabbit mAb #69953, two distinct bands are typically observed in western blotting experiments. These bands represent two different isoforms of the ANKRD15 protein: 

  • Isoform 1 is predominantly expressed in heart and kidney tissues
  • Isoform 2 is thought to be widely expressed at basic levels in various tissues
These isoforms are produced by alternative promoter usage and differ in size by approximately 20 kDa.

For more detailed information on the alternative splicing of ANKRD15 (also known as Kank), please refer to the following research article, which provides an in-depth analysis of the ANKRD15/Kank gene structure and its alternative splicing patterns:
  • Wang Y, Onishi Y, Kakinuma N, Roy BC, Aoyagi T, Kiyama R. Alternative splicing of the human Kank gene produces two types of Kank protein. Biochem Biophys Res Commun. 2005;330(4):1247-1253. doi:10.1016/j.bbrc.2005.03.106

Last updated: July 1, 2024

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