Why do you recommend ethanol fixation and HCl permeabilization for your BrdU, 5-hmC, and 5-mC antibodies?

These antibodies target a post-translational modification (PTM) on DNA rather than protein. In order to adequately expose the epitope for binding, the DNA needs to be partially denatured. To accomplish this, we use ethanol and HCl. Traditional formaldehyde fixation followed by detergent-based permeabilization methods will not work for these PTMs.

Last updated: September 12, 2024