Why do your Rad18 antibodies detect two bands by western blot?

We believe the upper band observed when using the Rad18 (D2B8) XP® Rabbit mAb #9040, Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb #14978, or Phospho-Rad18 (Ser403) Antibody #8393 is likely mono-ubiquitinated Rad18 (see Inagaki, A et al. (2011) PLoS One. 6(8), e23155 PMID: 21858012; https://www.ncbi.nlm.nih.gov/pubmed/21858012).  This target may also have other modifications, such as phosphorylation, due to two bands being seen with the phospho-antibodies.

Last updated: September 12, 2024