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Why does EGR1 run at 75kDa on a western blot when its predicted size is 58kDa?

Even though EGR1 has a predicted size of ~58kDa, 75kDa is the correct running size. This is because EGR1 is Sumoylated, which along with other PTMs, increases the size it runs at on a western blot. 

Last updated: September 12, 2024

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